DEVELOPMENT OF A POLYMERASE CHAIN REACTION TO DIFFERENTIATE AVIAN LEUKOSIS VIRUS (ALV) SUB-GROUPS: DETECTION OF AN ALV CONTAMINATE IN COMMERCIAL MAREK'S DISEASE VACCINES

2007 ◽  
Vol 2 (3) ◽  
pp. e6-e6
Author(s):  
ROBERT F. SILVA ◽  
ALY M. FADLY ◽  
SCOTT P. TAYLOR
Keyword(s):  
Polymerase Chain Reaction ◽  
Avian Leukosis Virus ◽  
Marek's Disease ◽  
Marek’S Disease ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Comparative suitability of the feathers, peripheral blood and spleen tissue samples of chickens for the detection of Marek’s disease virus by polymerase chain reaction

2019 ◽  
Vol 35 (1-2) ◽  
pp. 1-6
Author(s):  
MW Rahman ◽  
M Nooruzzaman ◽  
US Suma ◽  
EH Chowdhury ◽  
MR Islam
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Peripheral Blood ◽  
Buffy Coat ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Chain Reaction ◽  
Polymerase Chain

A total of 24 clinical specimens (10 feathers, 8 peripheral bloods and 6 spleens) were collected from 10 suspected outbreaks of Marek’s disease (MD) from Mymensingh, Tangail, Gazipur and Pabna districts of Bangladesh. A polymerase chain reaction (PCR) protocol originally described by Silva (1992) was adopted to detect Marek’s disease virus (MDV) genome in these specimens. All the tested peripheral blood buffy coat samples (100%) were positive for MDV in PCR, while 70% of feather samples and 66.6% of spleen samples were positive. A band of 317 bp size was found in all positive samples. A few samples also yielded additional bands of 185 bp size and/or multiple bands of larger than 317 bp size, indicating the presence of both virulent MDV and the vaccine virus. The study suggests that peripheral blood and feathers from live birds, and feathers from dead birds are the samples of choice for the detection of MDV by PCR. The Bangladesh Veterinarian (2018) 35(1&2): 1-6

scholarly journals DETECTION OF FIVE VIRUS INFECTIONS IN THE LAYER FARM WITH RUNTING-STUNTING SYNDROME IN SUKABUMI AND TANGERANG USING POLYMERASE CHAIN REACTION TECHNIQUE

2017 ◽  
Vol 11 (2) ◽  
Author(s):  
Risza Hartawan ◽  
Ni Luh Putu Indi Dharmayanti
Keyword(s):  
Polymerase Chain Reaction ◽  
Avian Influenza ◽  
Newcastle Disease ◽  
Viral Infections ◽  
Marek's Disease ◽  
Polymerase Chain Reaction Technique ◽  
Marek’S Disease ◽  
Chain Reaction ◽  
Field Samples ◽  
Polymerase Chain

The objective of this study was to identify the five different viral infections including avian influenza, Newcastle disease, avian reovirus, avian encephalomyelitis, and Marek’s disease in the runting-stunting syndrome outbreak in several commercial layer farms in Sukabumi and Tangerang in November 2014 using polymerase chain reaction technique. As results, this study identified mix infection of three viruses in the field samples, including Newcastle disease, reovirus, and avian encephalomyelitis; however, it was negative for avian influenza and Marek’s disease viruses. Subsequently, the inoculation of several samples into embryonated chicken eggs confirmed the growth of these three viruses. As a consequence, disease control management should be conducted in the affected farms by implementing effective biosecurity and vaccination program.

Sign in / Sign up

Export Citation Format

Share Document